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Abbkine Inc nuclear protein extraction kit
Nuclear Protein Extraction Kit, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclear+protein+extraction+kit/extraction+kit/pm42262027-82-6-11
Average 86 stars, based on 1 article reviews
nuclear protein extraction kit - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Isolation:

Article Title: Targeting the Mapk13‐Tcf1‐Slc7a5 Axis via One‐Carbon Metabolic Regulation to Prevent Chronic Allograft Vasculopathy
Article Snippet: The culture medium was supplemented with 1 μg/mL anti‐mouse CD28 antibodies (cat# BE0015, BioXcell). .. CD4 + T cells were isolated from spleen and stimulated with anti‐CD3/CD28 antibodies for 72 h. Cytoplasmic protein and nuclear protein separation were performed with Nuclear Protein Extraction Kit (cat# KTP3002, Abbkine) according to protocols. .. Subsequently, the protein lysate was incubated with primary antibodies or an IgG isotype antibody at 4°C for 12 h, followed by overnight incubation with Magna ChIP Protein A+G Magnetic Beads (Merck).

Article Title: NEDD8 Promotes the Progression and Inflammation of Keratoconus by Increasing the Expression of YAP1.
Article Snippet: Total proteins in corneal tissues and cells were extracted using RIPA lysis buffer (Solarbio Life Science) supplemented with phenylmethylsulfonyl fluoride (Solarbio Life Science), and a protein inhibitor cocktail (CWBiO). .. Nuclear proteins were isolated using the Nuclear Protein Extraction Kit (KTP3002; Abbkine, Wuhan, China).The proteins were quantified using a BCA protein assay kit. .. Protein samples were denatured at 95°C for 10 minutes, separated by SDS-PAGE and transferred to a 0.45 μm polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA).

Protein Extraction:

Article Title: Targeting the Mapk13‐Tcf1‐Slc7a5 Axis via One‐Carbon Metabolic Regulation to Prevent Chronic Allograft Vasculopathy
Article Snippet: The culture medium was supplemented with 1 μg/mL anti‐mouse CD28 antibodies (cat# BE0015, BioXcell). .. CD4 + T cells were isolated from spleen and stimulated with anti‐CD3/CD28 antibodies for 72 h. Cytoplasmic protein and nuclear protein separation were performed with Nuclear Protein Extraction Kit (cat# KTP3002, Abbkine) according to protocols. .. Subsequently, the protein lysate was incubated with primary antibodies or an IgG isotype antibody at 4°C for 12 h, followed by overnight incubation with Magna ChIP Protein A+G Magnetic Beads (Merck).

Article Title: The involvement of IRP2-induced ferroptosis through the p53-SLC7A11-ALOX12 pathway in Parkinson's disease.
Article Snippet: Disturbance in iron homeostasis has been described in Parkinson’s disease (PD), in which iron regulatory protein 2 (IRP2) plays a crucial role.. IRP2 deletion resulted in the misregulation of iron metabolism and subsequent neurodegeneration.. However, growing evidence showed that the levels of IRP2 were increased in the substantia nigra (SN) in MPTP-induced PD mice.

Article Title: NEDD8 Promotes the Progression and Inflammation of Keratoconus by Increasing the Expression of YAP1.
Article Snippet: Total proteins in corneal tissues and cells were extracted using RIPA lysis buffer (Solarbio Life Science) supplemented with phenylmethylsulfonyl fluoride (Solarbio Life Science), and a protein inhibitor cocktail (CWBiO). .. Nuclear proteins were isolated using the Nuclear Protein Extraction Kit (KTP3002; Abbkine, Wuhan, China).The proteins were quantified using a BCA protein assay kit. .. Protein samples were denatured at 95°C for 10 minutes, separated by SDS-PAGE and transferred to a 0.45 μm polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA).

Bicinchoninic Acid Protein Assay:

Article Title: NEDD8 Promotes the Progression and Inflammation of Keratoconus by Increasing the Expression of YAP1.
Article Snippet: Total proteins in corneal tissues and cells were extracted using RIPA lysis buffer (Solarbio Life Science) supplemented with phenylmethylsulfonyl fluoride (Solarbio Life Science), and a protein inhibitor cocktail (CWBiO). .. Nuclear proteins were isolated using the Nuclear Protein Extraction Kit (KTP3002; Abbkine, Wuhan, China).The proteins were quantified using a BCA protein assay kit. .. Protein samples were denatured at 95°C for 10 minutes, separated by SDS-PAGE and transferred to a 0.45 μm polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA).



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